Identification of an l-serine/l-threonine dehydratase with glutamate racemase activity in mammals
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منابع مشابه
Production of D-Glutamate from L-Glutamate with Glutamate Racemase and L-Glutamate Oxidase.
We studied production of D-glutamate from L-glutamate using a bioreactor consisting of two columns of sequentially connected immobilized glutamate racemase (EC 5.1.1.3, from Bacillus subtilis IFO 3336) and L-glutamate oxidase (EC 1.4.3.11, from Streptomyces sp. X119-6): L-glutamate was racemized by the glutamate racemase column, and then L-glutamate was oxidized by the L-glutamate oxidase colum...
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A timeand concentration-dependent irreversible inactivation of the Escherichia coli biodegradative threonine dehydratase by glyoxylate is accompanied by enzyme aggregation, apparent covalent binding of 4 mol of glyoxylate/mol of aggregated species, and a displacement of the absorption maximum of the enzyme-bound pyridoxal phosphate from 413 to 388 nm (Park, L. S., and Datta, P. (1979) J Biol. C...
متن کاملMolecular Evolution of Threonine Dehydratase in Bacteria
Threonine dehydratase converts L-threonine to 2-ketobutyrate. Several threonine dehydratases exist in bacteria, but their origins and evolutionary pathway are unknown. Here we analyzed all the available threonine dehydratases in bacteria and proposed an evolutionary pathway leading to the genes encoding three different threonine dehydratases CTD, BTD1 and BTD2. The ancestral threonine dehydrata...
متن کاملIsolation and Properties of a Homogeneous Preparation of Cystathionine Synthetase-l-serine and L-threonine Dehydratase.
Selim and Greenberg (1, 2) achieved a considerable degree of purification of L-serine dehydratase (L-serine hydro-lyase (deaminating), EC 4.2.1.13) from rat liver and demonstrated that this protein preparation contained the cystathionine-synthesizing activity of the liver (L-serine hydro-lyase (adding L-homocysteine), EC 4.2.1.21). These workers (2) also observed activity of their enzyme prepar...
متن کاملThreonine synthetase of Bacillus subtilis. The nature of an associated dehydratase activity.
The gene encoding threonine synthetase has been located by genetic transformation in Bacillus subtilis. Its position is adjacent and central to the genes encoding homoserine dehydrogenase and homoserine kinase. Its mutational sites are closely linked to, and interspersed among, several mutational sites affecting a minor but phenotypically significant threonine dehydratase (distinct from the maj...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 2020
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bcj20200721